Train drivers on the Victoria Line are to go on strike following a falling out with London Underground (LU) for “breaking promises”.
Members of the Rail, Maritime and Transport (RMT) union will walk out for 24 hours from 22:00 on 27 November.
The line is one of the busiest on the Tube network, carrying hundreds of thousands of passengers a day.
The union warned it would consider further strikes in December if the dispute was not resolved.
RMT general secretary Mick Cash accused LU management of reneging on agreements reached during talks.
Abuses of procedures, pay arrangements and constant harassment of staff were also were at the heart of the dispute, he added.
“It is extraordinary that LU seriously believed that they could get away with mugging off drivers on the Victoria Line by making promises and then pulling them away the moment that they step out into the daylight.
“LU’s actions are deliberately provocative and the announcement of action later this month is solely down to their childish behaviour.
“I have informed LU that the union remains available for talks to resolve this matter, but such talks have to be genuine, honest and based on mutual respect and trust.”
Transport for London has been approached for comment.
London Underground lines have been partially suspended as climate change protesters climbed on top of trains.
The Jubilee Line and Docklands Light Railway were affected after incidents at Stratford, Canning Town station and Shadwell during Tuesday’s rush hour.
Commuters were seen physically dragging protesters from the roof of a train in a video on social media.
Extinction Rebellion said the disruption was “necessary to highlight the emergency”.
British Transport Police said on Twitter: “At this time we have three separate incidents involving Extinction Rebellion protest action at Stratford, Canning Town, and Shadwell.
“Arrests have already been made and officers are working quickly to resume services.”